ASCP's Newest Book: Flow Cytometry in Clinical Diagnosis
The new edition of Flow Cytometry in Clinical Diagnosis promises a completely current overview of this increasingly commonplace technology. With every chapter either completely reworked or significantly updated to include the latest developments, and new contributions from 10 new international authorities, this will be the go-to resource on clinical flow cytometry for years to come. More than that, it promises to deliver a uniquely engaging learning experience with a bonus CD filled with real-world cases and interpretations.

The new book offers in-depth discussions from many of today’s leaders about how they are applying the technology in critical clinical areas.

• Dr. Philip McCoy presents a comprehensive overview of multiplex bead array assays, an exciting new technology that enables quantitation of soluble factors in serum and blood.
• Drs. Teri Oldaker and Elizabeth Stone, who were instrumental in the recent DNA Cytometry Consensus Conference, discuss quality control and quality assurance in clinical flow cytometry
• Drs. Robert A. Bray and Howard Gebel, whose recent research has set the standard of practice for flow cytometric crossmatching and antibody identification in the U.S., contribute a report on clinical utility of flow cytometry in allogeneic transplantation.
• Drs. Steven H. Kroft and Nitin J. Karandikar, who have published widely on their extensive studies on the potential utility of flow cytometry in the diagnosis of leukemias and lympomas, offer their insights about Flow Cytometric Analysis of Acute Leukemias, Myelodysplastic Syndromes and Myeloproliferative Disoders.

This 4th edition of Flow Cytometry in Clinical Diagnosis has been extensively revised to reflect recent advances in clinical flow cytometry. These include both technical advances (e.g., immunophenotyping with five or more markers and multiplex bead array assays) as well as new applications of existing assays (e.g., flow cytometry cross-match in transplantation; immunophenotypic analysis in the diagnosis of myelodysplasia or B CLL prognosis). The emphasis of this text continues to be clinically relevant applications of flow cytometry for the individual patient.

To realize these goals, 10 new authors have been added, in addition to 10 returning contributors from the 3rd edition. The 1st section of the book (chapters 1 through 7) provides basic technical and managerial information about flow cytometry (both traditional cellular assays and newer bead-based assays), monoclonal antibodies, comprehensive quality control, flow cytometry software, and the economics of flow cytometry. The second section in chapters 8 through 10 reviews the general and specific issues regarding flow immunophenotypic evaluation of hematolymphoid neoplasia, in the context of modern classification schemes. In addition to the written material, several dozen cases complete with list mode data files and corresponding expert interpretation are included on a companion CD-ROM.

The remainder of the book discuses the clinical applications of flow cytometry in a wide variety of settings. Chapters 11 and 12 review applications of flow cytometry to the diagnosis of congenital immuno-deficiencies of both the adaptive and innate immune systems, while chapter 13 reviews advances in qualitative and quantitative flow analysis of lymphoid cells in HIV-infected individuals. Chapter 14 provides an expert review of the applications of flow cytometry of anti-HLA antibodies and HLA antigens in the evaluation of organ transplant recipients and donors. Closely related is the review of methodologies for stem cell quantitation, and possible new assays in chapter 15. Chapter 16 reviews a mixture of other assays of non-neoplastic hematolymphoid cells (e.g., neutrophil activation; platelet function, fetal red cell quantitation), and, chapter 17 presents the clinical state of DNA analysis of tumors.

Lastly, the book compiles an extensive appendix of normal ranges for a wide variety of common antigens. These include 95th percentile confidence intervals for the most common markers for cord, neonatal, pediatric and adult peripheral blood samples, pediatric and adult bone marrows, and non-neoplastic (reactive) lymph nodes.

To purchase this book now, visit the ASCP's Online Store.